Search results for "Multiphoton microscopy"

showing 3 items of 3 documents

Cholesterol facilitates interactions between α‐synuclein oligomers and charge‐neutral membranes

2015

AbstractOligomeric species formed during α-synuclein fibrillation are suggested to be membrane-disrupting agents, and have been associated with cytotoxicity in Parkinson’s disease. The majority of studies, however, have revealed that the effect of α-synuclein oligomers is only noticeable on systems composed of anionic lipids, while the more physiologically relevant zwitterionic lipids remain intact. We present experimental evidence for significant morphological changes in zwitterionic membranes containing cholesterol, induced by α-synuclein oligomers. Depending on the lipid composition, model membranes are either unperturbed, disrupt, or undergo dramatic morphological changes and segregate …

AmyloidParkinson's diseaseFluorescent DyeBiophysicsPlasma protein bindingBiochemistryOligomerProtein Structure SecondaryMultiphoton microscopyMembrane phase separationCell membranechemistry.chemical_compoundGeneticStructural Biology2-NaphthylamineLaurdan fluorescenceGeneticsFluorescence microscopemedicineMolecular BiologyFluorescent DyesLaurateα-SynucleinMembranesChemistryMedicine (all)2-NaphthylamineCell MembraneMembraneCell BiologySettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)CholesterolMembranemedicine.anatomical_structureBiophysicBiochemistryStructural biologyOligomeralpha-SynucleinParkinson’s diseaseProtein MultimerizationLaurdanLauratesProtein BindingFEBS Letters
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Advanced fluorescence microscopy for in vivo imaging of neuronal activity

2019

Brain function emerges from the coordinated activity, over time, of large neuronal populations placed in different brain regions. Understanding the relationships of these specific areas and disentangling the contributions of individual neurons to overall function remain central goals for neuroscience. In this scenario, fluorescence microscopy has been proved as the tool of choice for in vivo recording of brain activity. Optical advances combined with genetically encoded indicators allow a large flexibility in terms of spatiotemporal resolution and field of view while keeping invasiveness in living animals to a minimum. Here we describe the latest advancements in the field of linear and nonl…

Flexibility (engineering)0303 health sciencesBrain activity and meditationComputer science01 natural sciencesAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materials010309 optics03 medical and health scienceslight-sheet microscopy; field-of-view; cellular-resolution; adaptive optics; multiphoton microscopy; GRID CELLS; HIGH-SPEED; LONG-TERM; 2-PHOTON; DEEPLight sheet fluorescence microscopy0103 physical sciencesFluorescence microscopePremovement neuronal activityIn vivo microscopyOptics In vivo imaging MicroscopyNeurosciencePreclinical imagingBrain function030304 developmental biologyOptica
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Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis.

2021

Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical micr…

MaleNonlinear Optical MicroscopyHyperkeratosisautofluorescencelcsh:Chemical technologyBiochemistryAnalytical Chemistry030207 dermatology & venereal diseases03 medical and health sciences0302 clinical medicineIn vivoKeratinmedicineHumanslcsh:TP1-1185Electrical and Electronic EngineeringInstrumentationkeratinSkinchemistry.chemical_classificationHyperkeratosis Epidermolyticintegumentary systemhyperkeratosisChemistryCommunicationOptical ImagingLEDnonlinear microscopyepidermolytic ichthyosismedicine.diseasediffuse reflectanceFluorescenceNonlinear optical microscopyAtomic and Molecular Physics and OpticsAutofluorescencemedicine.anatomical_structure030220 oncology & carcinogenesisChild Preschoolmultiphoton microscopyhistopathologyKeratinsKRT1EpidermisDiffuse reflectionBiomedical engineeringSensors (Basel, Switzerland)
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